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Marrow-Derived Stromal Cell Delivery on Fibrin Microbeads Can Correct Radiation-Induced Wound Healing Deficits

Skin that is exposed to radiation has an impaired ability to heal wounds. This is especially true for

whole body irradiation, where even moderate non-lethal doses can result in wound healing

deficits. Our previous attempts to administer dermal cells locally to wounds to correct radiationinduced

deficits were hampered by poor cell retention. Here we improve the outcome by using

biodegradable fibrin microbeads (FMB) to isolate a population of mesenchymal marrow-derived

stromal cells (MSC) from murine bone marrow by their specific binding to the fibrin matrix,

culture them to high density in vitro and deliver them as MSC on FMB at the wound site. MSC are

retained and proliferate locally and assist wounds gain tensile strength in whole body irradiated

mice with or without additional skin only exposure. MSC-FMB were effective in 2 different

mouse strains but were ineffective across a major histocompatability barrier. Remarkably,

irradiated mice whose wounds were treated with MSC-FMB showed enhanced hair regrowth

suggesting indirect effect on the correction of radiation-induced follicular damage. Further studies

showed that additional wound healing benefit could be gained by administration of G-CSF and

AMD3100. Collagen strips coated with haptides and MSCs were also highly effective in

correcting radiation-induced wound healing deficits.


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